Human erythrocytes exposure to juglone leads to an increase of superoxide anion production associated with cytochrome b5 reductase uncoupling
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چکیده
منابع مشابه
Membrane-bound cytochrome b5 reductase (methemoglobin reductase) in human erythrocytes. Study in normal and methemoglobinemic subjects.
In this study we present evidence that in human erythrocytes NADH-cytochrome b5 reductase (methemoglobin reductase) is not only soluble but also tightly bound to the membrane. The membrane methemoglobin reductase-like activity is unmasked by Triton X-100 treatment, and represents about half of the total activity in the erythrocytes. Like the amphiphilic microsomal-bound cytochrome b5 reductase,...
متن کاملThe interaction of NADH-cytochrome b5 reductase and cytochrome b5 bound to egg lecithin liposomes.
Incubation of liposomes prepared by sonication of egg lecithin with the amphipathic form of cytochrome b5 results in the binding of a maximum of 244 molecules of cytochrome b5 per liposomal vesicle. Interactions of the phospholipid with the hydrophobic segment of cytochrome b5 are involved in this binding which does not disrupt the liposome. When a small amount of NADH-cytochrome b5 reductase i...
متن کاملCytochrome b5 reductase activity in erythrocytes and leukocytes as related to sex and age.
We measured cytochrome b5 reductase (EC 1.6.2.2) activity in erythrocytes and leukocytes from subjects of both sexes ranging in age from neonate to adulthood, all either apparently healthy or without hematologic disorders. The activities were determined spectrophotometrically (J. Lab. Clin. Med. 72: 339-344, 1968). Within-run precision (CV) of the reductase determination was 6.4% and 3.7% for e...
متن کاملCharacterization of the purified NADH-cytochrome b5 reductase of human erythrocytes as a FAD-containing enzyme.
NADH-cytochrome b5 reductase of normal human erythrocytes was purified by procedures including affinity chromatography on Blue-Sepharose to an electrophoretically homogeneous protein. The purified enzyme was judged to be a typical flavoprotein based on its absorption spectrum (absorption maxima, 272, 390, and 462 nm; shoulders, 373 and 488 nm) and flavin content (1 mol of FAD/mol of enzyme). Th...
متن کاملImproved determination of cytochrome b5 in human erythrocytes.
A sensitive, precise enzymic/spectrophotometric method for determining cytochrome b5 in small amounts of blood is described. Mean values for healthy individuals, ages 20 to 70 years, were 0.26 (SD 0.03) mumol per liter of erythrocytes or 0.87 (SD 0.14) nmol per gram of hemoglobin. We believe the assay is preferable to methods described hitherto, primarily because of its high sensitivity.
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ژورنال
عنوان ژورنال: Biochimica et Biophysica Acta (BBA) - Bioenergetics
سال: 2020
ISSN: 0005-2728
DOI: 10.1016/j.bbabio.2019.148134